Background: In today’s evolving workforce, women play pivotal roles in all sectors, yet face unique challenges in balancing their professional and personal lives. Issues such as workplace inequality, lack of family support, and limited organizational policies contribute to stress and work-life imbalance among women professionals, particularly in healthcare settings.

Methods: A quantitative, descriptive design was adopted. A total of 347 working women at IMS and SUM Hospital, Bhubaneswar, were selected using a purposive sampling technique. Data were collected using structured tools on socio-demographics, determinants of work-life balance, and workplace challenges. Analysis was conducted using descriptive and inferential statistics.

Results: Findings revealed that 71.8% of participants experienced mild determinants affecting work-life balance, 27.7% faced maximum determinants, and only 0.6% had none. Regarding challenges, 95.4% faced mild challenges, 0.9% reported maximum challenges, and 3.7% faced none. Significant associations were found between determinants and factors such as age, religion, occupation of the family head, and job concessions during pregnancy. Monthly family income and adequate sleep were significantly associated with challenges.